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Tom Clancy's Splinter Cell: Conviction introduces several new gameplay mechanics that differentiate it from its predecessors. The most notable change is the removal of the radar system, which forces players to rely on their wits and observational skills to navigate the game's environments. Fisher can now use his surroundings to take out enemies silently, using objects like bottles, pipes, and even his own body to dispatch foes.

The game follows the story of Sam Fisher, a former Third Echelon operative who has retired from active duty and is now raising his daughter, Sophie. However, his quiet life is disrupted when terrorists murder his daughter in a public bombing. Fisher is consumed by grief and a desire for revenge. He uses his skills to track down the terrorists and uncover the conspiracy behind the bombing.