Within queer spaces, conversations about (the specific prejudice against trans women that combines transphobia and misogyny) have become central. This framework helps explain why trans women face higher rates of violence and sexual assault than trans men or non-binary people.

features several prominent characters who identify outside the gender binary or have transitioned, though their portrayals differ significantly across arcs: Kikunojo (Kiku) : Explicitly identified as a woman "at heart,"